C-DOCTOR Imaging Resources

USC Microscopy Core Facility – Seth Ruffins (ruffins@usc.edu)
Confocal and fluorescence microscopy; slide scanning services

USC Molecular Imaging Center – Peter Conti (pconti@usc.edu)
In vitro & in vivo evaluation of molecular targets, probe validation, bio-distribution studies, small animal imaging (PET, SPECT, MR, optical, ultrasound, photoacoustics, CT), dosimetry studies, large animal imaging; novel probe development through custom synthesis, peptide synthesis, custom conjugation to fluorescent dyes & microbubbles; radio-labeling; porosity; process devleopment & validation; drug product formulation & stability testing; cGMP production of PET imaging bio-markers

UCSF 3T MRI – Sergey Magnitsky (sergey.magnitsky@ucsf.edu)
The Bruker 3T scanner is specially designed for pre-clinical MRI studies of rats and mice. A special dual tuned 13C-1H RF coil is available for hyperpolarized DNP studies using 13C labelled biomarkers. The scanner is equipped with a cryogen free magnet and high performance (90G/cm) gradients.

UCSF Quantitative Micro-Imaging – Galateia Kazakia (galateia.kazakia@ucsf.edu)
The Quantitative Micro-Imaging Facility is equipped to provide both ex- and in-vivo micro-tomography (µCT). Using both a desktop specimen scanner, and a human extremity scale scanner, we have the capability to perform Bone Morphometry, Skeletal Phenotyping, Cancer and Vascular Imaging, and Osteoporosis and Rheumatoid Arthritis Imaging. We can also perform Fourier Transform Infrared Imaging (FTIR).

UCSF microCT and FTIR – Wenhan Chang (wenhan.chang@ucsf.edu)
This core provides services in microCT imaging for small animals and tissue specimens. This imaging system, manufactured by Scanco Medical, utilizes a Scanco VivaCT40 scanner for live animal imaging and a Scanco µCT50 scanner dedicated to specimen imaging.

UCSF MicroPET/CT and MicroSPECT/CT – Youngho Seo (youngho.seo@ucsf.edu)
Nuclear imaging solutions utilizing dual modality microPET/CT, microSPECT/CT.Our Nuclear-Optical Imaging Core is fully equipped with pre-clinical imaging instrumentation, live subject housing and a surgical suite, biodistribution gamma counter, full-body cryotome, and autoradiography.

UCSF Radiopharmaceutical Facility – James Slater (jim.slater@ucsf.edu)
Manufacture PET drugs for routine clinical studies. Manufacture PET Drugs for investigational clinical and animal studies. Provide PET drugs and isotopes for basic science research. Provide assistance in protocol design using radiopharmaceuticals and pharmaceuticals.

Stanford Center for Innovation in In-Vivo Imaging – Tim Doyle (timbo@stanford.edu) or Laura Jean Pisani (lpisani@stanford.edu)
IVIS imaging system; micro CT; Vevo ultrasound; Leica Cellvizio microscope; cryomicrotome systems; micro PET; 7T MRI; Art Optix; CRi Maestro fluorescence imaging system; imaging quantification

UCLA Microscopy Core – Luisa Iruela-Arispe (arispe@mbi.ucla.edu)
Includes 2-photon confocal and fluorescence microscopes

UCLA Advanced Light Microscopy/Spectroscopy and Macroscale Imaging Facilities – Shimon Weiss (sweiss@chem.ucla.edu)
The facility includes a Inverted Leica TCS-SP8-SMD Confocal Microscope, a Widefield Leica DMRXA upright microscope, Leica TCS SP2 AOBS filter-free spectral confocal microscope, Confocal and Multiphoton Leica TCS SP2 MP AOBS microscope system, a Inverted Leica TCS-SP5 AOBS Confocal Microscope, Leica TCS-SP5 AOBS Confocal Multiphoton STED microscope, A home-built single-molecule microscopy set-up for alternating laser excitation spectroscopy, a Leica DMI6000 inverted microscope, CRi Maestro™ 2 in vivo small animal Imaging System, Nikon TE2000E inverted microscope and Leica system for laser microdissection.

UCLA Electron Imaging Center for Nanomachines – Hong Zhou (hong.zhou@ucla.edu)
Electron microscopes, computer processing, sample preparation, and EM tomography services.

UCLA Nano & Pico Characterization Lab – James Gimzewski (gimzewski@cnsi.ucla.edu)
Nano-scale surface analysis instrumentation for the visualization and analysis of surfaces, adsorbates, nanostructures and devices at the atomic, molecular and cellular scales. Also provides training, sample analysis and consulting.

UCLA Brain Research Institute Microscopic Techniques Lab – Marianne Cilluffo (mariannc@ucla.edu)
Leica TCS-SP8 confocal microscope, wide field fluorescence microscope dedicated to FISH (fluorescence in situ hybridization) imaging; a home-built system for ALEX (alternating laser excitation spectroscopy); macroscale imaging, one upright and one inverted microscope set up for microinjection, fluorescence wide field time lapse (inverted) and multispectral unmixing (upright)

UCLA Preclinical Imaging Technology Center – Jason Lee (jasontlee@mednet.ucla.edu)
The Imaging Center offers microPET, microCT, bioluminescence and fluorescence imaging modalities and complementary in vitro/ex vivo services including cell-based assays, biodistribution, digital autoradiography and dosimetry. Companion PET tracer radiochemistry and radiolabeling services are available in-house and is supported by on-campus cyclotron facilities.

UCLA Crump Cyclotron & Radiochemistry Technology Center – Michael van Dam (mvandam@mednet.ucla.edu)
The Crump Cyclotron and Radiochemistry Technology Center houses a cyclotron for production of PET radioisotopes (e.g. [F-18]fluoride), as well as radiochemistry lab space and analytical equipment for characterization and testing of radiochemistry-related technologies, development of new radiolabeling strategies, development of novel PET tracers, and routine production of PET tracers for preclinical imaging. The facility is fully-equipped for research and production of [F-18]-labeled tracers. It houses one 11 MeV negative ion cyclotron (RDS-111 Eclipse HP, Siemens), four hot-cells (von Gahlen), six mini-cells (von Gahlen), one custom-built radioisotope aliquotting system, four semi-preparative radio-HPLCs (Knauer) w/ gamma detectors (Bioscan), seven dose calibrators (CRC-25 PET, Capintec), two automated radiosynthesizers (ELIXYS, Sofie Biosciences), two microwave reactors (Discovery, CEM), a custom-made remotely-operated radiochemistry system (Crump Radiochemistry System), a Cerenkov imaging system for monitoring radioactivity handling in microfluidic chips, and numerous radiochemistry technology projects under development. In addition, there is a dedicated analytical chemistry area, containing one radio-TLC scanner (mini-Gita, Raytest), one gas chromatography system (with mass spectrometry detector) (GC-MS) (7890A GC w/5975 MSD, Agilent), and two analytical radio-HPLC systems (one Waters and one Knauer) equipped with gamma detectors.

UCLA X-ray Crystallography Core Facility – Duilio Cascio (cascio@mbi.ucla.edu)
Evaluation of sample via Dynamic Light Scattering. Automatic setup of 4000 crystallization conditions per hour using 500uL of sample. (Hanging or Sitting Drops) Capable of distinguishing between organic and inorganic crystals using a sophisticated UV/vis microscope.

UCLA-DOE Crystallization Core – Michael Collazo (mcollazo@mbi.ucla.edu)
Experimental and computational facilities for X-ray based structure analysis and refinement, and for supporting structure determination by crystallography, and computational methods. Acquisition of X-ray diffraction data using in-house high brilliance generators.