FAQs

Stanford Medicinal Chemistry Knowledge Center – Mark Smith (mxsmith@stanford.edu) Provides help to biologists and clinicians to incorporate medicinal chemistry into their research endeavors Stanford Macromolecular Structure Knowledge Center – Marc Deller (mdeller@stanford.edu) Production, purification, and characterization of biological macromolecules Stanford Metabolic Chemistry Analysis Center – Curt Fischer (curtf@stanford.edu) Measurement of metabolites UCLA Biological Chemistry Imaging Facility Fluorescent scanning equipment and gel documentation systems UCLA Protein Expression Technology Center – Mark Arbing (marbing@mbi.ucla.edu) All aspects of protein expression from cloning through expression optimization; purification of proteins for structure/function studies UCLA-DOE & Biochemistry Instrumentation Core Facility – Martin Phillips (mlphill@ucla.edu) Molecular weight determination, kinetic & thermodynamic analysis of ligand binding, structural characterization, gel documentation & analysis, radioisotope detection & quantification, and spectroscopy UCLA Analytical Phytochemical Core – Qing-Yi Lu (qlu@mednet.ucla.edu) Quantitative analysis of biological active ingredients in fruits, vegetables, beverages, botanicals and dietary supplements; preparation and standardization of plant extract; measurement of in vivo and human blood and tissue absorption of phytochemicals (or therapeutic drugs); characterization of metabolites from blood, tissue, urinary and fecal samples; quantification of chemical carcinogens, contaminants, toxins in food and dietary supplements, and other chemicals such as short (C2-C7) and long chain fatty acids, cholesterol, bile acids in biological samples and amino acids using HPLC, GC, LC-MS methods. UCLA Bio-NMR Core – Robert Peterson (peterson@mbi.ucla.edu) High-field NMR instrumentation UCLA Bioscience Synthetic Chemistry Core – Michael Jung (jung@chem.ucla.edu) Synthesis of small organic molecules UCLA Mass Spectrometry & Proteomics Lab – Gregory Khitrov (khitrov@chem.ucla.edu) Wide range of sample characterization techniques UCLA Metabolomics Shared Resource – Daniel Braas (dbraas@mednet.ucla.edu) Study metabolism with a particular focus on central carbon metabolism (glycolysis, pentose phosphate pathway, TCA cycle, nucleos(t)ide synthesis, etc.). Metabolite analysis can be performed on a variety of specimens (e.g. cultured cells, culture medium, blood, urine, tissue). UCLA Pasarow Mass Spectrometry Lab – Kym Faull (faull@chem.ucla.edu) Consultation, training, and access to and technical assistance for metabolomics, proteomics (top-down and bottom-up) and targeted small molecule quantitation using mass spectrometery and contemporary chromatography UCLA Peptide Synthesis Core Facility Can synthesize isotope-edited versions of proteins and peptides suitable for detailed determinations of 3-D molecular structure by NMR spectroscopy or X-ray diffraction. UCSF Mass Spectrometry Facility – Al Burlingame (alb@cgl.ucsf.edu) Post-translational protein characterization

Stanford Quantitative Sciences Unit Biostatistics, informatics, clinical research methods Stanford Protégé Ontology framework helps build knowledge-based solutions in areas as diverse as biomedicine, e-commerce, and organizational modeling. National Center for Biomedical Ontology – Nigam Shah (nigam@stanford.edu) Repository and web services for ontologies UCLA Bioinfomatics & Computational Core Technology Center – Sabeeha Merchant (merchant@chem.ucla.edu) UCLA Bioinformatics Core UCLA Microbiome Center Bioinformatics Core –  Jennifer Labus (jlabus@ucla.edu)

Stanford Functional Genomics Facility – John Coller (microarray@stanford.edu) High-throughput sequencing (Illumina), library generation, microarrays (Affymetrix, Agilent, Illumina), whole-genome sequencing (Illumina), real-time PCR, genotyping, melt, etc.; sample prep, DNA & RNA extraction, plasmic prep, NanoString UCLA Technology Center for Genomics & Bioinformatics – Xinmin Li (xinminli@mednet.ucla.edu) Fully automated, high-throughput genomic facility equipped with all major next generation sequencing and microarray platforms UCLA Neuroscience Genomics Core – Joe DeYoung (jdeyoung@icnn.ucla.edu) Currently operating an Illumina BeadLab 1000 high throughput SNP genotyping system (iScan), a Sequenom MassArray Compact mass spec and and two Illumina HiSeq 2500 next generation sequencing instruments UCLA High Throughput Screening – Suhua Feng (sfeng@mcdb.ucla.edu) UCSF Gladstone Genomics Core – Nathasha Carli (genomics-lab@gladstone.ucsf.edu) The mission of the Gladstone Genomics Core is to provide genome-wide analysis for clients interested in gene expression, regulation of gene expression, and genome sequence and variation. The primary forms of genome-wide analysis are the Affymetrix GeneChip microarray, Illumina MiSeq next generation sequencing, and Fluidigm Realtime PCR technologies. In addition to providing experimental expertise for performing microarray and high-throughput DNA sequencing, the staff provides advice and consultation on experimental design, and general analysis approaches for microarray and high-throughput DNA sequencing-based research.  

USC – Michael Jamieson (mjamieso@usc.edu)

UC Davis Center for Laboratory Animal Science – William Ferrier (wtferrier@ucdavis.edu) Large and small animal models USC Department of Animal Resources – James Finlay (jbfinlay@med.usc.edu) Large and small animals USC Transgenic/Knockout Rodent Core Facility – Nancy Lanying Wu (nwu@usc.edu) Transgenic & knockout mice, CRISPR, knockout rats UCLA Zebrafish Core – Shuo Lin (shuolin@ucla.edu) Transgenesis, large-scale genetic screens UCLA Mouse Physiology Lab – Kenneth Roos (kroos@mednet.ucla.edu) Electrocardiography, hemodynamics, surgical procedures, telemetric recording, autonomic testing, exercise & metabolic testing, postmortem morphometry, isolated cell studies, certification testing of new ddrugs UCLA Behavioral Testing Core – Michael Fanselow (fanselow@psych.ucla.edu) Behavioral testing of mice and rats UCLA Center for AIDS Research Humanized Mouse Core – Scott Kitchen (skitchen@ucla.edu) Humanized immunodeficient mice

USC Microscopy Core Facility – Seth Ruffins (ruffins@usc.edu) Confocal and fluorescence microscopy; slide scanning services USC Molecular Imaging Center – Peter Conti (pconti@usc.edu) In vitro & in vivo evaluation of molecular targets, probe validation, bio-distribution studies, small animal imaging (PET, SPECT, MR, optical, ultrasound, photoacoustics, CT), dosimetry studies, large animal imaging; novel probe development through custom synthesis, peptide synthesis, custom conjugation to fluorescent dyes & microbubbles; radio-labeling; porosity; process devleopment & validation; drug product formulation & stability testing; cGMP production of PET imaging bio-markers UCSF 3T MRI – Sergey Magnitsky (sergey.magnitsky@ucsf.edu) The Bruker 3T scanner is specially designed for pre-clinical MRI studies of rats and mice. A special dual tuned 13C-1H RF coil is available for hyperpolarized DNP studies using 13C labelled biomarkers. The scanner is equipped with a cryogen free magnet and high performance (90G/cm) gradients. UCSF Quantitative Micro-Imaging – Galateia Kazakia (galateia.kazakia@ucsf.edu) The Quantitative Micro-Imaging Facility is equipped to provide both ex- and in-vivo micro-tomography (µCT). Using both a desktop specimen scanner, and a human extremity scale scanner, we have the capability to perform Bone Morphometry, Skeletal Phenotyping, Cancer and Vascular Imaging, and Osteoporosis and Rheumatoid Arthritis Imaging. We can also perform Fourier Transform Infrared Imaging (FTIR). UCSF microCT and FTIR – Wenhan Chang (wenhan.chang@ucsf.edu) This core provides services in microCT imaging for small animals and tissue specimens. This imaging system, manufactured by Scanco Medical, utilizes a Scanco VivaCT40 scanner for live animal imaging and a Scanco µCT50 scanner dedicated to specimen imaging. UCSF MicroPET/CT and MicroSPECT/CT – Youngho Seo (youngho.seo@ucsf.edu) Nuclear imaging solutions utilizing dual modality microPET/CT, microSPECT/CT.Our Nuclear-Optical Imaging Core is fully equipped with pre-clinical imaging instrumentation, live subject housing and a surgical suite, biodistribution gamma counter, full-body cryotome, and autoradiography. UCSF Radiopharmaceutical Facility – James Slater (jim.slater@ucsf.edu) Manufacture PET drugs for routine clinical studies. Manufacture PET Drugs for investigational clinical and animal studies. Provide PET drugs and isotopes for basic science research. Provide assistance in protocol design using radiopharmaceuticals and pharmaceuticals. Stanford Center for Innovation in In-Vivo Imaging – Tim Doyle (timbo@stanford.edu) or Laura Jean Pisani (lpisani@stanford.edu) IVIS imaging system; micro CT; Vevo ultrasound; Leica Cellvizio microscope; cryomicrotome systems; micro PET; 7T MRI; Art Optix; CRi Maestro fluorescence imaging system; imaging quantification UCLA Microscopy Core – Luisa Iruela-Arispe (arispe@mbi.ucla.edu) Includes 2-photon confocal and fluorescence microscopes UCLA Advanced Light Microscopy/Spectroscopy and Macroscale Imaging Facilities – Shimon Weiss (sweiss@chem.ucla.edu) The facility includes a Inverted Leica TCS-SP8-SMD Confocal Microscope, a Widefield Leica DMRXA upright microscope, Leica TCS SP2 AOBS filter-free spectral confocal microscope, Confocal and Multiphoton Leica TCS SP2 MP AOBS microscope system, a Inverted Leica TCS-SP5 AOBS Confocal Microscope, Leica TCS-SP5 AOBS Confocal Multiphoton STED microscope, A home-built single-molecule microscopy set-up for alternating laser excitation spectroscopy, a Leica DMI6000 inverted microscope, CRi Maestro™ 2 in vivo small animal Imaging System, Nikon TE2000E inverted microscope and Leica system for laser microdissection. UCLA Electron Imaging Center for Nanomachines – Hong Zhou (hong.zhou@ucla.edu) Electron microscopes, computer processing, sample preparation, and EM tomography services. UCLA Nano & Pico Characterization Lab – James Gimzewski (gimzewski@cnsi.ucla.edu) Nano-scale surface analysis instrumentation for the visualization and analysis of surfaces, adsorbates, nanostructures and devices at the atomic, molecular and cellular scales. Also provides training, sample analysis and consulting. UCLA Brain Research Institute Microscopic Techniques Lab – Marianne Cilluffo (mariannc@ucla.edu) Leica TCS-SP8 confocal microscope, wide field fluorescence microscope dedicated to FISH (fluorescence in situ hybridization) imaging; a home-built system for ALEX (alternating laser excitation spectroscopy); macroscale imaging, one upright and one inverted microscope set up for microinjection, fluorescence wide field time lapse (inverted) and multispectral unmixing (upright) UCLA Preclinical Imaging Technology Center – Jason Lee (jasontlee@mednet.ucla.edu) The Imaging Center offers microPET, microCT, bioluminescence and fluorescence imaging modalities and complementary in vitro/ex vivo services including cell-based assays, biodistribution, digital autoradiography and dosimetry. Companion PET tracer radiochemistry and radiolabeling services are available in-house and is supported by on-campus cyclotron facilities. UCLA Crump Cyclotron & Radiochemistry Technology Center – Michael van Dam (mvandam@mednet.ucla.edu) The Crump Cyclotron and Radiochemistry Technology Center houses a cyclotron for production of PET radioisotopes (e.g. [F-18]fluoride), as well as radiochemistry lab space and analytical equipment for characterization and testing of radiochemistry-related technologies, development of new radiolabeling strategies, development of novel PET tracers, and routine production of PET tracers for preclinical imaging. The facility is fully-equipped for research and production of [F-18]-labeled tracers. It houses one 11 MeV negative ion cyclotron (RDS-111 Eclipse HP, Siemens), four hot-cells (von Gahlen), six mini-cells (von Gahlen), one custom-built radioisotope aliquotting system, four semi-preparative radio-HPLCs (Knauer) w/ gamma detectors (Bioscan), seven dose calibrators (CRC-25 PET, Capintec), two automated radiosynthesizers (ELIXYS, Sofie Biosciences), two microwave reactors (Discovery, CEM), a custom-made remotely-operated radiochemistry system (Crump Radiochemistry System), a Cerenkov imaging system for monitoring radioactivity handling in microfluidic chips, and numerous radiochemistry technology projects under development. In addition, there is a dedicated analytical chemistry area, containing one radio-TLC scanner (mini-Gita, Raytest), one gas chromatography system (with mass spectrometry detector) (GC-MS) (7890A GC w/5975 MSD, Agilent), and two analytical radio-HPLC systems (one Waters and one Knauer) equipped with gamma detectors. UCLA X-ray Crystallography Core Facility – Duilio Cascio (cascio@mbi.ucla.edu) Evaluation of sample via Dynamic Light Scattering. Automatic setup of 4000 crystallization conditions per hour using 500uL of sample. (Hanging or Sitting Drops) Capable of distinguishing between organic and inorganic crystals using a sophisticated UV/vis microscope. UCLA-DOE Crystallization Core – Michael Collazo (mcollazo@mbi.ucla.edu) Experimental and computational facilities for X-ray based structure analysis and refinement, and for supporting structure determination by crystallography, and computational methods. Acquisition of X-ray diffraction data using in-house high brilliance generators.

USC/SC CTSI – Kathleen Rodgers (krodgers@usc.edu) Medical device safety, drug effectiveness, toxicological testing UCLA Molecular Screening Shared Resource – Robert Damoiseaux (rdamoiseaux@mednet.ucla.edu) High throughput screening with a total of roughly 200,000 compounds in various libraries, siRNA sets of the druggable genome for mouse and human and a database of results from screens.

USC Stevens Center for Innovation – Mina Zion (mzion@usc.edu) Technology transfer, IP protection, commercialization, corporate collaborations Stanford – Michael Longaker (longaker@stanford.edu) UCLA Technology Development Group – Amir Naiberg (amir.naiberg@tdg.ucla.edu)

USC Regulatory Science – Michael Jamieson (jamieso@usc.edu) Regulatory science and clinical research planning SC CTSI – Nicki Karimipour (nicki.karimipour@med.usc.edu) Clinical research support, informatics, biostatistics, clinical trial management, regulatory science UC BRAID

City of Hope Biological & Cellular GMP Manufacturing Facility – David Hsu (dhsu@coh.org) or Joseph Gold (jogold@coh.org) The Center for Biomedicine & Genetics (CBG) is a California-licensed, 20, 000 square foot, multi-product biologics manufacturing facility. With twelve ISO 7 production rooms in three product type “zones”, a dedicated aseptic fill suite and a staff with extensive biopharmaceutical experience, the CBG is capable of producing virtually any type of biologic at scales suitable for Phase I through Phase II clinical trials. The Cellular Therapies Production Center (CTPC) is a 6,800 square foot cell therapy manufacturing facility comprising six ISO 7 production rooms. The CTPC supports the production of manipulated autologous and allogeneic cell therapies. UC Davis GMP Lab – Gerhard Bauer (gerhard.bauer@ucdavis.edu) UC Davis’ Good Manufacturing Practice facility in Sacramento features six manufacturing rooms with Class 10,000, multi-use cleanroom capabilities. It also offers an associated product scale-up and testing lab. Unique features include a GMP-grade FACS sorter, switchable positive-negative room pressurization for gene therapy vector manufacturing, and a hot cell for clinical grade PET reagent manufacturing. Stanford Laboratory for Cell & Gene Medicine – David DiGiusto (digiusto@stanford.edu) To produce individualized therapies for phases one and two clinical trials. UCLA Janis V. Georgi Flow Cytometry Core Facility – Zoran Galic (zgalic@ucla.edu) Instrumentation and technical and professional assistance for performing laser-based analytic flow cytometry, image cytometry and cell sorting, as well as mass cytometry. The facility operates one three laser BD-LSRFortessa X-20 analyzer, two five-laser BD LSRII analyzers, one three-laser BD-LSR II analyzer with a high throughput option, one ImageStreamx MarkII imaging flow cytometer, and for cell sorting, three BD FACSAria high-speed cell sorters, a Helios (a CYTOF system) mass cytometer and a RoboSep, an automated immunomagnetic bead cell separator from STEMCELL Technologies UCLA Flow Cytometry Core – Felicia Codrea (fcodrea@mednet.ucla.edu) UCLA hESC and IPS Cell Core – Jinghua Tang (jinghuatang@mednet.ucla.edu) or Saravanan Karumbayaram (skarumbayaram@mednet.ucla.edu) Culturing of hESCs and IPS cells UCLA Cellular Bioenergetics Core – Laurent Vergnes (lvergnes@ucla.edu) Measure of cellular respiration and glycolysis from cells, mitochondria, tissues, worm and yeast. UCLA Immune Assessment Core – Maura Rossetti (mrossetti@mednet.ucla.edu) Standardized and customizable multi-parameter flow cytometry, immunoassays and functional assays for assessing various immune cell functions, including T cells, B cells, NK cells, monocytes and granulocytes. UCLA Immuno/BioSpot Core – Brent Gordon (brgordon@ucla.edu) Detection, quantification, and qualification of biological spot assays and single-cell related analyses, including ELISPOT, colony counting, plaque assays, FluoroSpot, cell viability tests, apoptosis tests, in vivo/in vitro cytotoxicity measurements using cells labeled with fluorescent dyes, histochemistry stains, genotoxicity assays, multi-color intracellular/surface quantification, etc. UCLA Human Gene & Cell Therapy Program & GMP Facility – Vance Handley (vhandley@mednet.ucla.edu) Clinical cell and gene processing laboratories infrastructure; supports documentation and monitoring oversight of gene and cell product manufacture